Selected article for: "electron microscope and uranyl acetate"
Author: Whitehouse, Caroline; Burchell, Joy; Gschmeissner, Stephen; Brockhausen, Inka; Lloyd, Kenneth O.; Taylor-Papadimitriou, Joyce
Title: A Transfected Sialyltransferase That Is Elevated in Breast Cancer and Localizes to the medial/trans-Golgi Apparatus Inhibits the Development of core-2–based O-Glycans
  • Document date: 1997_6_16
    • ID: pwgj97kz_27
    Snippet: Cells were fixed for 1 h at room temperature in 0.1% glutaraldehyde/4% paraformaldehyde before being scraped, spun, and stored overnight in 2% paraformaldehyde at 4 Њ C. Double labeling was performed as described previously (Slot et al., 1991) . Antibody details are as follows: the grids were incubated overnight at 4 Њ C with 9E10 supernatant, diluted 1:5, followed, after washing, for 30 min with goat anti-mouse Ig conjugated to 10-nm gold part.....
    Document: Cells were fixed for 1 h at room temperature in 0.1% glutaraldehyde/4% paraformaldehyde before being scraped, spun, and stored overnight in 2% paraformaldehyde at 4 Њ C. Double labeling was performed as described previously (Slot et al., 1991) . Antibody details are as follows: the grids were incubated overnight at 4 Њ C with 9E10 supernatant, diluted 1:5, followed, after washing, for 30 min with goat anti-mouse Ig conjugated to 10-nm gold particles. Galactosyltransferase rabbit polyclonal antibody (Watzele et al., 1991) was diluted 1:50 in 1% BSA, and the grids were incubated for 30 min at room temperature before washing followed by a 30min incubation with protein A coupled to 5-nm gold. The grids were embedded in 1.8% methyl cellulose/0.4% uranyl acetate before examination using a Zeiss 10C (Oberkochen, Germany) or JEOL 1010 (JEOL USA, Peabody, MA) electron microscope.

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