Author: Whitehouse, Caroline; Burchell, Joy; Gschmeissner, Stephen; Brockhausen, Inka; Lloyd, Kenneth O.; Taylor-Papadimitriou, Joyce
Title: A Transfected Sialyltransferase That Is Elevated in Breast Cancer and Localizes to the medial/trans-Golgi Apparatus Inhibits the Development of core-2–based O-Glycans Document date: 1997_6_16
ID: pwgj97kz_48
Snippet: Sequencing the cytoplasmic tail, transmembrane domain, and part of the stalk region of endogenous â£2,3 sialyltransferase, prepared by PCR from T47D and MTSV1-7 (see Materials and Methods), showed that the sequence was identical to the transfected enzyme (data not shown). Thus, the transfected enzyme contains the same sequence that determines the residency of the endogenous â£2,3 sialyltransferase in the Golgi apparatus......
Document: Sequencing the cytoplasmic tail, transmembrane domain, and part of the stalk region of endogenous â£2,3 sialyltransferase, prepared by PCR from T47D and MTSV1-7 (see Materials and Methods), showed that the sequence was identical to the transfected enzyme (data not shown). Thus, the transfected enzyme contains the same sequence that determines the residency of the endogenous â£2,3 sialyltransferase in the Golgi apparatus.
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