Author: Stenglein, Mark D.; Jacobson, Elliott R.; Wozniak, Edward J.; Wellehan, James F. X.; Kincaid, Anne; Gordon, Marcus; Porter, Brian F.; Baumgartner, Wes; Stahl, Scott; Kelley, Karen; Towner, Jonathan S.; DeRisi, Joseph L.
Title: Ball Python Nidovirus: a Candidate Etiologic Agent for Severe Respiratory Disease in Python regius Document date: 2014_9_9
ID: rb3qdunj_11
Snippet: Molecular diagnostics. Pathology and EM findings were consistent with a viral etiology, so reverse transcription-PCR (RT-PCR) was used to attempt to identify an etiologic agent. Consensus primers targeted viruses in the families Paramyxoviridae (genus Ferlavirus), Rhabdoviridae, Reoviridae (genera Orthoreovirus and Aquareovirus), and Filoviridae (15) (16) (17) (18) (19) . RNA was extracted from frozen lung tissue from animals 1 and 6 and used as .....
Document: Molecular diagnostics. Pathology and EM findings were consistent with a viral etiology, so reverse transcription-PCR (RT-PCR) was used to attempt to identify an etiologic agent. Consensus primers targeted viruses in the families Paramyxoviridae (genus Ferlavirus), Rhabdoviridae, Reoviridae (genera Orthoreovirus and Aquareovirus), and Filoviridae (15) (16) (17) (18) (19) . RNA was extracted from frozen lung tissue from animals 1 and 6 and used as a template for reactions, which were uniformly negative, though positive-control primers targeting conserved host sequences were positive.
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