Author: Lu, Yan; Jiang, Yun; Ling, Lijun; Zhang, Yunyi; Li, Hong; Chen, Daofeng
Title: Beneficial effects of Houttuynia cordata polysaccharides on “two-hit” acute lung injury and endotoxic fever in rats associated with anti-complementary activities Document date: 2017_12_8
ID: q4r36tpw_21
Snippet: The upper lobe of right lung was fixed immediately in 4% formaldehyde. After the routine paraffin embedding, the sample was cut into 5-μm sections and stained with haematoxylin and eosin (H&E) for the detection of lung histopathological alteration. The severity of the lung injury was blindly quantified by a pathologist based on microscopic changes, including extent of disease, alveolar wall thickness, exudation, pulmonary congestion and inflamma.....
Document: The upper lobe of right lung was fixed immediately in 4% formaldehyde. After the routine paraffin embedding, the sample was cut into 5-μm sections and stained with haematoxylin and eosin (H&E) for the detection of lung histopathological alteration. The severity of the lung injury was blindly quantified by a pathologist based on microscopic changes, including extent of disease, alveolar wall thickness, exudation, pulmonary congestion and inflammation degree. The injury was graded from 0 (normal) to 4 (severe) in the above categories. The deposit of complement activation products was determined using immunohistochemical staining with rabbit anti-human C3c 20 .
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