Author: Fuqua, Joshua L.; Hamorsky, Krystal; Khalsa, Guruatma; Matoba, Nobuyuki; Palmer, Kenneth E.
Title: Bulk production of the antiviral lectin griffithsin Document date: 2015_7_14
ID: yaqioaa5_8
Snippet: After production and initial qualification, the plasmid bank is stored at À20°C and placed on a stability monitoring program ( Table 1 ). The stability monitoring program for the plasmid bank includes monitoring sequence stability and functionality. Sequence stability is monitored by double-strand sequencing the GRFT sequence within the plasmid, and functionality is monitored by transcribing the plasmid and assuring the naked TMV RNA remains in.....
Document: After production and initial qualification, the plasmid bank is stored at À20°C and placed on a stability monitoring program ( Table 1 ). The stability monitoring program for the plasmid bank includes monitoring sequence stability and functionality. Sequence stability is monitored by double-strand sequencing the GRFT sequence within the plasmid, and functionality is monitored by transcribing the plasmid and assuring the naked TMV RNA remains infectious, using the infectivity metric of symptomology. It is assumed the plasmid bank will remain stable for >2 years, but this needs to be validated empirically using the proposed methods to understand if and when the plasmid bank might fail.
Search related documents:
Co phrase search for related documents- double strand and plasmid bank: 1
- GRFT sequence and plasmid bank: 1
Co phrase search for related documents, hyperlinks ordered by date