Cell free preparations of probiotics exerted antibacterial and antibiofilm activities against multidrug resistant E. coli

Abdelhamid, Ahmed G.; Esaam, Aliaa; Hazaa, Mahmoud M.

In this work, six multidrug resistant E. coli were isolated. They were designated as E. coli IC1 to E. coli IC4, E. coli WW1 to E. coli WW2 as they were isolated from the intensive care unit and sewage water, respectively. All isolates were resistant to at least five antibiotics (Ceftazidime, Ampicillin, Clarithromycin, Amoxicillin + Clavulanic Acid and Ceftriaxone). Only one isolate showed no resistance to Cefoxitin. In addition, three isolates were intermediate in sensitivity to amikacin while two isolates exhibited intermediate sensitivity to cefoxitin and ciprofloxacin. These results demonstrated that the multidrug resistance pattern was exhibited by all isolates of which E. coli WW2 and E. coli IC2 were the most resistant to all antibiotics and were subjected to antibiofilm experiments. The antibiotic resistance patterns were summarized in Table 2 .