Author: Xing, Liang; Sun, Feng; Wang, Zhendong; Li, Yan; Yang, Zhifang; Wang, Fengshan; Zhai, Guangxi; Tan, Haining
Title: Characterization and bioactivity of self-assembled anti-angiogenic chondroitin sulfate-ES2-AF nanoparticle conjugate Document date: 2019_4_10
ID: x8f598x2_3
Snippet: The CS-TBA was prepared according to previously described protocols. 10, 17, 18 Then, the CS-TBA was dissolved in DMSO and BOP (1 g) was added and mixed for 30 minutes to activate the carboxyl groups of CS-TBA. The ES2-AF peptide (11 mg) , dissolved in DMSO, was added to the reaction solution. Finally, DIPEA (50 μL) was added to the reaction solution and mixed at 37°C. After 24 hours, an equal volume of 1 M NaCl aqueous solution was added to th.....
Document: The CS-TBA was prepared according to previously described protocols. 10, 17, 18 Then, the CS-TBA was dissolved in DMSO and BOP (1 g) was added and mixed for 30 minutes to activate the carboxyl groups of CS-TBA. The ES2-AF peptide (11 mg) , dissolved in DMSO, was added to the reaction solution. Finally, DIPEA (50 μL) was added to the reaction solution and mixed at 37°C. After 24 hours, an equal volume of 1 M NaCl aqueous solution was added to the reaction solution, the pH was adjusted to 3.0 with 1 M HCl to terminate the reaction, and then the pH was raised to 7.0 with 1 M NaOH. The resulting solution was dialyzed in a dialysis bag (5 kDa molecular weight cutoff) and subsequently freeze-dried. The structure of the obtained CS-ES2-AF was determined by 1 H-NMR and stored at 2°C-8°C until further analysis.
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