Author: Longhini, Andrew P.; LeBlanc, Regan M.; Becette, Owen; Salguero, Carolina; Wunderlich, Christoph H.; Johnson, Bruce A.; D'Souza, Victoria M.; Kreutz, Christoph; Dayie, T. Kwaku
Title: Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations Document date: 2016_4_7
ID: uhhtvdif_2
Snippet: However, in spite of this importance, directly establishing how dynamics modulates the structure and function of RNAs has been difficult because X-ray crystallography and nuclear magnetic resonance spectroscopy are plagued by distinct but equally challenging problems. In crystallography, motions can only be observed in the ps-ns timescales and the strain imposed by crystal packing can obscure and distort structural data (9, 10) ......
Document: However, in spite of this importance, directly establishing how dynamics modulates the structure and function of RNAs has been difficult because X-ray crystallography and nuclear magnetic resonance spectroscopy are plagued by distinct but equally challenging problems. In crystallography, motions can only be observed in the ps-ns timescales and the strain imposed by crystal packing can obscure and distort structural data (9, 10) .
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