Author: Viitanen, S.J.; Lappalainen, A.; Rajamäki, M.M.
Title: Co-infections with Respiratory Viruses in Dogs with Bacterial Pneumonia Document date: 2015_3_27
ID: qkw27nfk_12
Snippet: Hematology, serum biochemistry, arterial blood gas analysis, serum CRP, and fecal analysis as well as cytologic and microbiologic analysis of respiratory samples were performed as previously described. 48 Semiquantitative bacterial culture of BALF and TTW fluid was performed as follows: A 10-lL volume of the specimen was streaked onto agars. Bacterial counts were quantified by calculating the colonies on a plate and multiplying the number of colo.....
Document: Hematology, serum biochemistry, arterial blood gas analysis, serum CRP, and fecal analysis as well as cytologic and microbiologic analysis of respiratory samples were performed as previously described. 48 Semiquantitative bacterial culture of BALF and TTW fluid was performed as follows: A 10-lL volume of the specimen was streaked onto agars. Bacterial counts were quantified by calculating the colonies on a plate and multiplying the number of colonies by the dilution factor. The limit of detection was 100 colony-forming units/mL. The remaining respiratory samples (BALF, TTW) and serum were immediately frozen and stored at À80°C. CPIV, CAV-2, CHV, CIV, CDV, and CRCoV, as well as Mycoplasma spp. and B. bronchiseptica, were analyzed by real-time PCR analysis at a commercial reference laboratory. b 6 CnPnV was analyzed by RT-PCR at the laboratory c responsible for the discovery of this pathogen. 25
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