Author: Al-Mulla, Hawaa M. N.; Turrell, Lauren; Smith, Nicola M.; Payne, Luke; Baliji, Surendranath; Züst, Roland; Thiel, Volker; Baker, Susan C.; Siddell, Stuart G.; Neuman, Benjamin W.
Title: Competitive Fitness in Coronaviruses Is Not Correlated with Size or Number of Double-Membrane Vesicles under Reduced-Temperature Growth Conditions Document date: 2014_4_1
ID: tfuupgkg_14
Snippet: The number of DMVs per cell was measured in order to better quantify differences in organelle prevalence. Brts31 DMV clusters contained about a three-quarters as many DMVs as wild-type clusters both early and late in infection (Table 3) . Albts22 clusters contained about two-thirds as many DMVs as wild-type clusters, consistent with the severely attenuated growth. For all viruses examined, there was one CM present per approximately 7 DMVs and 1 t.....
Document: The number of DMVs per cell was measured in order to better quantify differences in organelle prevalence. Brts31 DMV clusters contained about a three-quarters as many DMVs as wild-type clusters both early and late in infection (Table 3) . Albts22 clusters contained about two-thirds as many DMVs as wild-type clusters, consistent with the severely attenuated growth. For all viruses examined, there was one CM present per approximately 7 DMVs and 1 to 5 DMV clusters per cell section, when present (data not shown). Collectively these data distinguish two low-DMV phenotypes that occurred without attenuation of the virus: Brts105 formed fewer DMVs of near-normal size, while Brts31 formed fewer DMVs of smaller-than-normal size.
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