Author: Al-Mulla, Hawaa M. N.; Turrell, Lauren; Smith, Nicola M.; Payne, Luke; Baliji, Surendranath; Züst, Roland; Thiel, Volker; Baker, Susan C.; Siddell, Stuart G.; Neuman, Benjamin W.
Title: Competitive Fitness in Coronaviruses Is Not Correlated with Size or Number of Double-Membrane Vesicles under Reduced-Temperature Growth Conditions Document date: 2014_4_1
ID: tfuupgkg_31
Snippet: Measurement of intracellular features was done using the boxer module of EMAN (46) . The longest and shortest diameters of each DMV and intracellular virus particle were measured twice each by two observers. The standard deviation between repeated measurements was about 2 pixels on average, which equated to approximately 7 nm at the level of the specimen. Since most of the DMVs and virions had an elliptical profile, size was estimated by Ramanuja.....
Document: Measurement of intracellular features was done using the boxer module of EMAN (46) . The longest and shortest diameters of each DMV and intracellular virus particle were measured twice each by two observers. The standard deviation between repeated measurements was about 2 pixels on average, which equated to approximately 7 nm at the level of the specimen. Since most of the DMVs and virions had an elliptical profile, size was estimated by Ramanujan's first approximation for the perimeter of ellipse, and the result was divided by to approximate diameter.
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