Selected article for: "complete sequence and consensus assembly"
Author: Malboeuf, Christine M.; Yang, Xiao; Charlebois, Patrick; Qu, James; Berlin, Aaron M.; Casali, Monica; Pesko, Kendra N.; Boutwell, Christian L.; DeVincenzo, John P.; Ebel, Gregory D.; Allen, Todd M.; Zody, Michael C.; Henn, Matthew R.; Levin, Joshua Z.
Title: Complete viral RNA genome sequencing of ultra-low copy samples by sequence-independent amplification
  • Document date: 2012_9_8
    • ID: s76c5ebd_4
    Snippet: In this study, we evaluated the use of a sequence-independent RNA amplification method, NuGEN's Ovation RNA-Seq system, for capturing complete target regions of viral genomes from low-copy HIV, respiratory syncytial virus (RSV) and WNV samples. Previously, this amplification method was used for cellular transcriptome analysis (26, 27) with input amounts of 500 pg to 100 ng of total RNA. Here, using only femtograms to attograms of viral RNA with t.....
    Document: In this study, we evaluated the use of a sequence-independent RNA amplification method, NuGEN's Ovation RNA-Seq system, for capturing complete target regions of viral genomes from low-copy HIV, respiratory syncytial virus (RSV) and WNV samples. Previously, this amplification method was used for cellular transcriptome analysis (26, 27) with input amounts of 500 pg to 100 ng of total RNA. Here, using only femtograms to attograms of viral RNA with this amplification method in combination with Illumina sequencing and de novo assembly of viral reads, we successfully generated consensus sequence for the complete, or very nearly complete, CDS of viral genomes.

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