Author: Enora Dupas; Bruno Legendre; Valérie Olivier; Françoise Poliakoff; Charles Manceau; Amandine Cunty
Title: Comparison of real-time PCR and droplet digital PCR for the detection of Xylella fastidiosa in plants Document date: 2019_3_20
ID: 0xc02kkb_9
Snippet: As expected, in these assays, no cross-reaction was found, as no positive droplets were found 261 for any of the NTCs, for the plant matrices. With the exception of O. europaea (curve could not 262 be drawn since only two concentrations gave some results), all the ddPCR matrix standard 263 curves showed high linearity and amplification efficiency, as the correlation coefficient (R²) 264 was higher than 0.96. This indicated the successful outcome.....
Document: As expected, in these assays, no cross-reaction was found, as no positive droplets were found 261 for any of the NTCs, for the plant matrices. With the exception of O. europaea (curve could not 262 be drawn since only two concentrations gave some results), all the ddPCR matrix standard 263 curves showed high linearity and amplification efficiency, as the correlation coefficient (R²) 264 was higher than 0.96. This indicated the successful outcomes and good performances of all the 265 assays (Table 2) . 266 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/582288 doi: bioRxiv preprint
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