Author: Benharouga, Mohamed; Haardt, Martin; Kartner, Norbert; Lukacs, Gergely L.
Title: Cooh-Terminal Truncations Promote Proteasome-Dependent Degradation of Mature Cystic Fibrosis Transmembrane Conductance Regulator from Post-Golgi Compartments Document date: 2001_5_28
ID: q3agdeju_15
Snippet: BHK or COS-1 cells were pulse labeled in the presence of [ 35 S]methionine and [ 35 S]cysteine (0.1-0.25 mCi/ml; Amersham Pharmacia Biotech) and chased in complete medium for the specified time at 37 Њ C. CFTR was immunoprecipitated with a mixture of M3A7 and L12B4 anti-CFTR Abs, as described (Lukacs et al., 1994) . The duration of pulse and chase periods and withdrawals of samples are indicated on schematic figures. Radioactivity associated wit.....
Document: BHK or COS-1 cells were pulse labeled in the presence of [ 35 S]methionine and [ 35 S]cysteine (0.1-0.25 mCi/ml; Amersham Pharmacia Biotech) and chased in complete medium for the specified time at 37 Њ C. CFTR was immunoprecipitated with a mixture of M3A7 and L12B4 anti-CFTR Abs, as described (Lukacs et al., 1994) . The duration of pulse and chase periods and withdrawals of samples are indicated on schematic figures. Radioactivity associated with CFTR was visualized by fluorography and quantified by PhosphorImager (PDI) using the ImageQuant software (Haardt et al., 1999) .
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