Selected article for: "density gradient and percoll density"
Author: Benharouga, Mohamed; Haardt, Martin; Kartner, Norbert; Lukacs, Gergely L.
Title: Cooh-Terminal Truncations Promote Proteasome-Dependent Degradation of Mature Cystic Fibrosis Transmembrane Conductance Regulator from Post-Golgi Compartments
  • Document date: 2001_5_28
    • ID: q3agdeju_31
    Snippet: Pulse-chase analysis has revealed that the disposal of the complex-glycosylated wt CFTR was mitigated by NH 4 Cl and chloroquin (Fig. 2, E and F) . To further examine the discordant role of lysosomal proteases in the wt and T70 CFTR degradation, subcellular fractionation was performed, after the enrichment of short-lived proteolytic intermediates that may have been difficult to detect by treating the cells with the cathepsin inhibitors. Heavy and.....
    Document: Pulse-chase analysis has revealed that the disposal of the complex-glycosylated wt CFTR was mitigated by NH 4 Cl and chloroquin (Fig. 2, E and F) . To further examine the discordant role of lysosomal proteases in the wt and T70 CFTR degradation, subcellular fractionation was performed, after the enrichment of short-lived proteolytic intermediates that may have been difficult to detect by treating the cells with the cathepsin inhibitors. Heavy and light microsomes, containing predominantly lysosomes and Golgi complex plasma membrane, and endosomes, respectively, were isolated on Percoll density gradient (Fig. 3 A) .

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