Comparison of SARS-CoV-2 infections among 3 species of non-human primates

Shuaiyao Lu; Yuan Zhao; Wenhai Yu; Yun Yang; Jiahong Gao; Junbin Wang; Dexuan Kuang; Mengli Yang; Jing Yang; Chunxia Ma; Jingwen Xu; Haiyan Li; Siwen Zhao; Jingmei Li; Haixuan Wang; Haiting Long; Jingxian Zhou; Fangyu Luo; Kaiyun Ding; Daoju Wu; Yong Zhang; Yingliang Dong; Yuqin Liu; Yingqiu Zheng; Xiaochen Lin; Li Jiao; Huanying Zheng; Qing Dai; Qiangmin Sun; Yunzhang Hu; Changwen Ke; Hongqi Liu; Xiaozhong Peng

For ultrastructural sections, tissues were successively fixed in 2.5% glutaraldehyde and 1% osmium tetroxide solution. After gradual dehydration in various concentrations of ethanol, tissues were embedded in epoxy resin and cut into 60-70nm sections. After stained with 2% uranium acetate, ultrathin sections were examined under Hitachi transmission electron microscope H-7650. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08.031807 doi: bioRxiv preprint CoV-2 genome). FFPE slides were pretreated by baking, de-paraffinizing, target retrieval and protease treatment. SARS-CoV-2 specific probe was applied to the pretreated slides and hybridized with viral genome. Fluorophore was added to detect the hybridization on slides, followed by counter-staining with DAPI. Slides were scanned via 3D HISTECH system for further analysis. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08.031807 doi: bioRxiv preprint 7.72% ↓ HHH- 12 11.36% ↓ *Body weights (BW) of animals were measured on day 2 and day 6 post virus inoculation. Body weight change (%) was expressed as |BW on 6 dpi-BW on 2 dpi|/BW on 2 dpi.