Selected article for: "african green monkey and cell culture"
Author: Antas, Marta; Wozniakowski, Grzegorz
Title: Current Status of Porcine Epidemic Diarrhoea (PED) in European Pigs
  • Document date: 2019_10_24
    • ID: r2sjv9ih_8
    Snippet: Isolation of PEDV from field samples is very difficult. The virus grows in Vero cells (African green monkey kidney). However, its growth there requires the presence of trypsin-supplemented cell culture medium, because this protease plays an important role in cell entry and the release of PEDV virions in Vero cells. Trypsin does this by cleaving the S protein into S1 and S2 subunits, and it enables efficient replication and spread of PEDV in vitro.....
    Document: Isolation of PEDV from field samples is very difficult. The virus grows in Vero cells (African green monkey kidney). However, its growth there requires the presence of trypsin-supplemented cell culture medium, because this protease plays an important role in cell entry and the release of PEDV virions in Vero cells. Trypsin does this by cleaving the S protein into S1 and S2 subunits, and it enables efficient replication and spread of PEDV in vitro (18, 29) . PEDV adapted to the Vero cell line can be successfully propagated in other cell types. PED viruses cause cell death leading to lysis of infected cells, a change observable under the microscope as a cytopathic effect (CPE) which is characterised by cell membrane vacuolisation and syncytium formation (13, 15, 24) .

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