Author: Zhang, XiZhen; Wang, XiaoDan; Zhao, DongHai; Meng, XiangYu; Zhao, XingHong; Yu, XiangHui; Kong, Wei
Title: Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine Document date: 2011_12_16
ID: xxypq3wd_15
Snippet: Spleens from immunized mice were harvested two weeks after the fourth immunization. Approximately 1×10 6 P815/BALB target cells were sensitized with synthetic peptide at a concentration of 1 mmol L ï€1 , for 2 h at 37°C. Cytotoxicity was measured by a standard lactate dehydrogenase (LDH) release assay with a CTL kit (Promega, Madison, WI, USA) according to the manufacturer's instructions......
Document: Spleens from immunized mice were harvested two weeks after the fourth immunization. Approximately 1×10 6 P815/BALB target cells were sensitized with synthetic peptide at a concentration of 1 mmol L ï€1 , for 2 h at 37°C. Cytotoxicity was measured by a standard lactate dehydrogenase (LDH) release assay with a CTL kit (Promega, Madison, WI, USA) according to the manufacturer's instructions.
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