Author: Azhar, Esam I.; Hashem, Anwar M.; El-Kafrawy, Sherif A.; Sohrab, Sayed Sartaj; Aburizaiza, Asad S.; Farraj, Suha A.; Hassan, Ahmed M.; Al-Saeed, Muneera S.; Jamjoom, Ghazi A.; Madani, Tariq A.
Title: Detection of the Middle East Respiratory Syndrome Coronavirus Genome in an Air Sample Originating from a Camel Barn Owned by an Infected Patient Document date: 2014_7_22
ID: ynlj4cge_7
Snippet: Air sampling procedure. Air samples were collected from the camels' barn on three consecutive days, with day 1 (7 November 2013) being the same day that one of the nine camels was positive for MERS-CoV by real-time RT-PCR. Samples were collected using the MD8 airscan sampling device (Sartorius) and sterile gelatin filters (80 mm in diameter and 3-m pore size; type 17528-80-ACD; Sartorius). Air was sampled at a speed of 50 liters/min for 20 min. F.....
Document: Air sampling procedure. Air samples were collected from the camels' barn on three consecutive days, with day 1 (7 November 2013) being the same day that one of the nine camels was positive for MERS-CoV by real-time RT-PCR. Samples were collected using the MD8 airscan sampling device (Sartorius) and sterile gelatin filters (80 mm in diameter and 3-m pore size; type 17528-80-ACD; Sartorius). Air was sampled at a speed of 50 liters/min for 20 min. Filters were dissolved in 5 ml viral transport medium (VTM) and stored at Ϫ80°C until analyzed.
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