Author: PIEWBANG, Chutchai; RUNGSIPIPAT, Anudep; POOVORAWAN, Yong; TECHANGAMSUWAN, Somporn
Title: Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs Document date: 2016_9_15
ID: w3g6ohac_15
Snippet: Reproducibility: Both intra-and inter-assay variations were measured using the positive controls and sequenced clinical samples. To assess the intra-assay variation, triplicate amplifications of the 2 −10 and 2 0 ng/reaction templates for the positive controls and the samples were performed in a single multiplex PCR assay. To evaluate the inter-assay variation, the above single multiplex PCR was performed as three independent multiplex PCR assa.....
Document: Reproducibility: Both intra-and inter-assay variations were measured using the positive controls and sequenced clinical samples. To assess the intra-assay variation, triplicate amplifications of the 2 −10 and 2 0 ng/reaction templates for the positive controls and the samples were performed in a single multiplex PCR assay. To evaluate the inter-assay variation, the above single multiplex PCR was performed as three independent multiplex PCR assays.
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