Author: DOKI, Tomoyoshi; TAKANO, Tomomi; HOHDATSU, Tsutomu
Title: Development of a mouse-feline chimeric antibody against feline tumor necrosis factor-alpha Document date: 2016_6_4
ID: y0x0quha_26
Snippet: Chimeric mAb 2-4 was prepared by fusion of the variable region of anti-fTNF-alpha mouse mAb 2-4 and the feline antibody constant region and expressed in FO cells. Mouse mAb 2-4 and chimeric mAb 2-4 were purified and analyzed using western blotting. Anti-mouse IgG antibody reacted with mouse mAb 2-4 but did not react with chimeric mAb 2-4, whereas anti-feline IgG antibody did not react with mouse mAb 2-4 but reacted with chimeric mAb 2-4, confirmi.....
Document: Chimeric mAb 2-4 was prepared by fusion of the variable region of anti-fTNF-alpha mouse mAb 2-4 and the feline antibody constant region and expressed in FO cells. Mouse mAb 2-4 and chimeric mAb 2-4 were purified and analyzed using western blotting. Anti-mouse IgG antibody reacted with mouse mAb 2-4 but did not react with chimeric mAb 2-4, whereas anti-feline IgG antibody did not react with mouse mAb 2-4 but reacted with chimeric mAb 2-4, confirming that the antigenicity of chimeric mAb 2-4 is similar to that of feline IgG. When neutralization of fTNF-alpha by purified chimeric mAb 2-4 was investigated, chimeric mAb 2-4 neutralized recombinant and natural fTNF-alpha, similarly to mouse mAb 2-4. Based on these findings, chimeric mAb 2-4 possesses fTNF-alpha neutralization activity similar to that of mouse mAb 2-4 while maintaining the characteristics of feline IgG.
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