Selected article for: "cell culture and cetuximab proteolytic cleavage"
Author: Tan, Zhaoli; Gao, Lihua; Wang, Yan; Yin, Huihui; Xi, Yongyi; Wu, Xiaojie; Shao, Yong; Qiu, Weiyi; Du, Peng; Shen, Wenlong; Fu, Ling; Jia, Ru; Zhao, Chuanhua; Zhang, Yun; Zhao, Zhihu; Sun, Zhiwei; Chen, Hongxing; Hu, Xianwen; Xu, Jianming; Wang, Youliang
Title: PRSS contributes to cetuximab resistance in colorectal cancer
  • Document date: 2020_1_1
    • ID: tymoeyoo_20
    Snippet: In detail, the HT-29 cell culture supernatant produced the highest degree of cetuximab cleavage, followed by the supernatant of the moderately cetuximab-sensitive LoVo cells, whereas the degree of cleavage produced by the cell culture supernatant of the cetuximabsensitive DiFi cells was the lowest (Fig. 3 , B and C). We also tested cetuximab cleavage using cell culture supernatants of shPRSS1-1, shPRSS1-2, mock, and control HT-29 cells and LoVo c.....
    Document: In detail, the HT-29 cell culture supernatant produced the highest degree of cetuximab cleavage, followed by the supernatant of the moderately cetuximab-sensitive LoVo cells, whereas the degree of cleavage produced by the cell culture supernatant of the cetuximabsensitive DiFi cells was the lowest (Fig. 3 , B and C). We also tested cetuximab cleavage using cell culture supernatants of shPRSS1-1, shPRSS1-2, mock, and control HT-29 cells and LoVo cells. Cetuximab cleavage was significantly decreased in the PRSS1 expression knockdown cell lines compared with that in the mock and control cells (Fig. 3 , D to G, and fig. S2D ). We also assessed cetuximab cleavage using cell culture supernatants of PRSS1-overexpressing, mock, and control DiFi cells and LoVo cells. Conversely, the exo genous addition of PRSS1-enriched medium to the cells increased cetuximab cleavage (Fig. 3 , H to K, and fig. S2, E and F). The proteolytic cleavage patterns of cetuximab in the cell culture supernatants of PRSS1overexpressing cells and PRSS1-silenced cells again proved that PRSS1 in the cell culture supernatant cleaved cetuximab.

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