Author: Tan, Zhaoli; Gao, Lihua; Wang, Yan; Yin, Huihui; Xi, Yongyi; Wu, Xiaojie; Shao, Yong; Qiu, Weiyi; Du, Peng; Shen, Wenlong; Fu, Ling; Jia, Ru; Zhao, Chuanhua; Zhang, Yun; Zhao, Zhihu; Sun, Zhiwei; Chen, Hongxing; Hu, Xianwen; Xu, Jianming; Wang, Youliang
Title: PRSS contributes to cetuximab resistance in colorectal cancer Document date: 2020_1_1
ID: tymoeyoo_48
Snippet: To generate stable cell lines, human PRSS1 expression constructs encoding full-length PRSS1 fusion protein and mature PRSS1 fusion protein were generated by cloning PCR-amplified sequences into pMSCU-puro (Amersham Pharmacia Biotech). Lipofectamine 2000 (Invitrogen) was used for transfection. Two days after transfection, stable overexpression cell lines were selected in puromycin (1 ïg/ml) for 14 days. Pooled clones or single clones were screen.....
Document: To generate stable cell lines, human PRSS1 expression constructs encoding full-length PRSS1 fusion protein and mature PRSS1 fusion protein were generated by cloning PCR-amplified sequences into pMSCU-puro (Amersham Pharmacia Biotech). Lipofectamine 2000 (Invitrogen) was used for transfection. Two days after transfection, stable overexpression cell lines were selected in puromycin (1 ïg/ml) for 14 days. Pooled clones or single clones were screened by qPCR, standard immunoblotting, and ELISA.
Search related documents:
Co phrase search for related documents- cell line and fusion protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
- cell line and overexpression cell line: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- cell line and PCR amplify: 1, 2, 3
- cell line and pool clone: 1
- cell line and single clone: 1, 2, 3, 4, 5
- cell line and stable cell line: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- cell line and stable cell line generate: 1
- cell line and stable overexpression cell line: 1
- expression construct and fusion protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- expression construct and stable cell line: 1
- fusion protein and PCR amplify: 1
- fusion protein and stable cell line: 1, 2
- overexpression cell line and stable cell line: 1
- overexpression cell line and stable overexpression cell line: 1
- pool clone and single clone: 1
Co phrase search for related documents, hyperlinks ordered by date