Author: Tan, Zhaoli; Gao, Lihua; Wang, Yan; Yin, Huihui; Xi, Yongyi; Wu, Xiaojie; Shao, Yong; Qiu, Weiyi; Du, Peng; Shen, Wenlong; Fu, Ling; Jia, Ru; Zhao, Chuanhua; Zhang, Yun; Zhao, Zhihu; Sun, Zhiwei; Chen, Hongxing; Hu, Xianwen; Xu, Jianming; Wang, Youliang
Title: PRSS contributes to cetuximab resistance in colorectal cancer Document date: 2020_1_1
ID: tymoeyoo_48
Snippet: To generate stable cell lines, human PRSS1 expression constructs encoding full-length PRSS1 fusion protein and mature PRSS1 fusion protein were generated by cloning PCR-amplified sequences into pMSCU-puro (Amersham Pharmacia Biotech). Lipofectamine 2000 (Invitrogen) was used for transfection. Two days after transfection, stable overexpression cell lines were selected in puromycin (1 ïg/ml) for 14 days. Pooled clones or single clones were screen.....
Document: To generate stable cell lines, human PRSS1 expression constructs encoding full-length PRSS1 fusion protein and mature PRSS1 fusion protein were generated by cloning PCR-amplified sequences into pMSCU-puro (Amersham Pharmacia Biotech). Lipofectamine 2000 (Invitrogen) was used for transfection. Two days after transfection, stable overexpression cell lines were selected in puromycin (1 ïg/ml) for 14 days. Pooled clones or single clones were screened by qPCR, standard immunoblotting, and ELISA.
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