Author: Han, Baek-Sang; Jang, Ho-Young; Racine, Trina; Qiu, Xiangguo; Sin, Jeong-Im
Title: Purification and characterization of monoclonal IgG antibodies recognizing Ebola virus glycoprotein Document date: 2018_7_31
ID: v5oh0haa_29
Snippet: We next tested whether the IgG antibodies from 5 clones (C36-1, D11-3, D12-1, D34-2, and E140-2) might react with the native forms of GP expressed on the surface of HEK293-GP cells. For this testing, 1 µg of the purified IgG was reacted with HEK293-GP cells, as well as control HEK293 cells without GP expression, followed by flow cytometry. As shown in Fig. 3 , the IgG antibodies from clones D11-3, D12-1, and E140-2 showed positive reactivity to .....
Document: We next tested whether the IgG antibodies from 5 clones (C36-1, D11-3, D12-1, D34-2, and E140-2) might react with the native forms of GP expressed on the surface of HEK293-GP cells. For this testing, 1 µg of the purified IgG was reacted with HEK293-GP cells, as well as control HEK293 cells without GP expression, followed by flow cytometry. As shown in Fig. 3 , the IgG antibodies from clones D11-3, D12-1, and E140-2 showed positive reactivity to HEK293-GP cells but not to HEK293 cells. In contrast, the IgG antibodies from clones C36-1 and D34-2 displayed no reactivity to HEK293-GP cells. This result suggests that the IgG antibodies from clones D11-3, D12-1, and E140-2 may recognize the native forms of GP expressed on the cell surface.
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