Author: Rogers, J.; Schoepp, R.J.; Schröder, O.; Clements, T.L.; Holland, T.F.; Li, J.Q.; Li, J.; Lewis, L.M.; Dirmeier, R.P.; Frey, G.J.; Tan, X.; Wong, K.; Woodnutt, G.; Keller, M.; Reed, D.S.; Kimmel, B.E.; Tozer, E.C.
Title: Rapid discovery and optimization of therapeutic antibodies against emerging infectious diseases Document date: 2008_5_13
ID: xkx56h0o_47
Snippet: The purified antibodies were tested in plaque reduction neutralization tests (PRNTs) using a live SARS virus on the permissive Vero E6 cell line. The results are summarized in Table I and showed five antibodies exhibiting 50% neutralization activity at concentrations ranging from 0.59 to 21 mg/ml. All three of the antibodies that had blocked spike binding to Vero E6 cells (i.e. 4049Fab14, 3889Fab33, 3889Fab35) also showed neutralization in the PR.....
Document: The purified antibodies were tested in plaque reduction neutralization tests (PRNTs) using a live SARS virus on the permissive Vero E6 cell line. The results are summarized in Table I and showed five antibodies exhibiting 50% neutralization activity at concentrations ranging from 0.59 to 21 mg/ml. All three of the antibodies that had blocked spike binding to Vero E6 cells (i.e. 4049Fab14, 3889Fab33, 3889Fab35) also showed neutralization in the PRNTs. Two additional antibodies that had not blocked spike binding did neutralize SARS infection, suggesting an inhibitory mechanism other than direct blockage of receptor binding epitopes. One of these antibodies, 4049Fab28, was discovered using viral isolates as the antigen. 4049Fab14 and 3889Fab33 showed 80% neutralization at concentrations of ,0.7 and 4.75 mg/ml, respectively. 4049Fab14 exhibited the best neutralization activity and was chosen for the subsequent optimization studies.
Search related documents:
Co phrase search for related documents- additional antibody and plaque reduction neutralization test: 1
- additional antibody and reduction neutralization: 1, 2
- additional antibody and SARS infection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- additional antibody and SARS virus: 1, 2, 3
- additional antibody and spike bind: 1
- antigen viral isolate and viral isolate: 1
- bind epitope and cell line: 1
- bind epitope and receptor bind epitope: 1, 2
- bind epitope and SARS infection: 1, 2, 3, 4
- bind epitope and SARS virus: 1, 2
- bind epitope and spike bind: 1, 2
- cell line and inhibitory mechanism: 1, 2
- cell line and mg ml concentration: 1, 2, 3, 4
- cell line and neutralization activity: 1, 2, 3, 4, 5, 6
- cell line and neutralization show: 1, 2
- cell line and plaque reduction neutralization: 1, 2, 3, 4
- cell line and plaque reduction neutralization test: 1, 2
- cell line and purified antibody: 1, 2
- cell line and reduction neutralization: 1, 2, 3, 4, 5
Co phrase search for related documents, hyperlinks ordered by date