Selected article for: "bovine serum and eagle medium"
Author: Lan, Jiaming; Yao, Yanfeng; Deng, Yao; Chen, Hong; Lu, Guangwen; Wang, Wen; Bao, Linlin; Deng, Wei; Wei, Qiang; Gao, George F.; Qin, Chuan; Tan, Wenjie
Title: Recombinant Receptor Binding Domain Protein Induces Partial Protective Immunity in Rhesus Macaques Against Middle East Respiratory Syndrome Coronavirus Challenge()
  • Document date: 2015_8_18
    • ID: q8i2gfut_7
    Snippet: MERS-CoV strain (hCoV-EMC/2012) was kindly provided by Professor Fouchier (Erasmus Medical Centre, Netherlands). MERS-CoV seed stocks were propagated in Vero cells (ATCC Number: CCL-81) without mycoplasma contamination maintained in Dulbecco's Modified Eagle Medium (DMEM, Invitrogen) supplemented with 10% foetal bovine serum (FBS), 100 international units (IU)/mL penicillin, and 100 μg/mL streptomycin, cultured at 37°C in 5% CO 2 . Seed stocks .....
    Document: MERS-CoV strain (hCoV-EMC/2012) was kindly provided by Professor Fouchier (Erasmus Medical Centre, Netherlands). MERS-CoV seed stocks were propagated in Vero cells (ATCC Number: CCL-81) without mycoplasma contamination maintained in Dulbecco's Modified Eagle Medium (DMEM, Invitrogen) supplemented with 10% foetal bovine serum (FBS), 100 international units (IU)/mL penicillin, and 100 μg/mL streptomycin, cultured at 37°C in 5% CO 2 . Seed stocks were diluted to the desired titre and used to determine the MERS-CoV 50% tissue culture infection dose (TCID 50 ), and for neutralising antibody and virus inoculation assays.

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