Author: Lamborn, Ian T.; Jing, Huie; Zhang, Yu; Drutman, Scott B.; Abbott, Jordan K.; Munir, Shirin; Bade, Sangeeta; Murdock, Heardley M.; Santos, Celia P.; Brock, Linda G.; Masutani, Evan; Fordjour, Emmanuel Y.; McElwee, Joshua J.; Hughes, Jason D.; Nichols, Dave P.; Belkadi, Aziz; Oler, Andrew J.; Happel, Corinne S.; Matthews, Helen F.; Abel, Laurent; Collins, Peter L.; Subbarao, Kanta; Gelfand, Erwin W.; Ciancanelli, Michael J.; Casanova, Jean-Laurent; Su, Helen C.
Title: Recurrent rhinovirus infections in a child with inherited MDA5 deficiency Document date: 2017_7_3
ID: vipx6t7e_40
Snippet: The lentiviral transfer vector pLenti-III-UbC/mCherry was generated by replacing the puromycin resistance cDNA in pLenti-III-UbC with mCherry cDNA from pLenti-PGK-mCherry (both from Applied Biological Materials). The mCherry cDNA was PCR-amplified with primers that appended 15 bp that are homologous with the pLenti vector. pLenti-III-UbC/Puro was linearized by restriction digest with BsiWI. Homologous recombination of the linearized vector and mC.....
Document: The lentiviral transfer vector pLenti-III-UbC/mCherry was generated by replacing the puromycin resistance cDNA in pLenti-III-UbC with mCherry cDNA from pLenti-PGK-mCherry (both from Applied Biological Materials). The mCherry cDNA was PCR-amplified with primers that appended 15 bp that are homologous with the pLenti vector. pLenti-III-UbC/Puro was linearized by restriction digest with BsiWI. Homologous recombination of the linearized vector and mCherry cDNA was performed by mixing the vector, cDNA, and Cold Fusion Cloning kit master mix (System Biosciences) as per the manufacturer's protocol.
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