Author: FAZ, Mirna; MARTÍNEZ, José Simón; QUIJANO-HERNÁNDEZ, Israel; FAJARDO, Raúl
Title: Reliability of clinical diagnosis and laboratory testing techniques currently used for identification of canine parvovirus enteritis in clinical settings Document date: 2016_11_6
ID: z3in6bi2_4
Snippet: Dog's stool samples were obtained using rectal swabs, which were suspended in nuclease-free water and 200 µl of the homogenates, and were used for DNA extraction. The procedure was performed using the QIAamp® DNA Stool DNA extraction kit (QIAGEN, Mainz, Germany), following the manufacturer's instructions. All DNA samples were quantified using a Q5000 Quawell spectrophotometer (Quawell Technology, Inc., San Jose, CA, U.S.A.). 100 ng of DNA of ea.....
Document: Dog's stool samples were obtained using rectal swabs, which were suspended in nuclease-free water and 200 µl of the homogenates, and were used for DNA extraction. The procedure was performed using the QIAamp® DNA Stool DNA extraction kit (QIAGEN, Mainz, Germany), following the manufacturer's instructions. All DNA samples were quantified using a Q5000 Quawell spectrophotometer (Quawell Technology, Inc., San Jose, CA, U.S.A.). 100 ng of DNA of each sample were used for PCR reactions with 50 µl of final volume. Previously, a pair of primers was designed in our laboratory to amplify a 275 bp fragment, ParvoInt2FB (5′-TCAAGCAGATGGTGATCCAAG-3′) and ParvoInt2CR (5′-GGTACATTATTTAATGCAGTTA-3′) located at nucleotides 1,107-1,130 and 1,360-1,382 of the VP2 gene (GenBank accession number FJ0051962c).
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