Author: Tang, Hei-Man Vincent; Gao, Wei-Wei; Chan, Chi-Ping; Cheng, Yun; Chaudhary, Vidyanath; Deng, Jian-Jun; Yuen, Kit-San; Wong, Chun-Ming; Ng, Irene Oi-Lin; Kok, Kin-Hang; Zhou, Jie; Jin, Dong-Yan
Title: Requirement of CRTC1 coactivator for hepatitis B virus transcription Document date: 2014_11_10
ID: qtoygz6w_8
Snippet: Sampling of human liver tissues was performed as previously described (22) . Seven Chinese patients who had surgical resection of liver at Queen Mary Hospital of Hong Kong were randomly selected for study. All specimens were obtained immediately after surgical resection, snap-frozen in liquid nitrogen and stored at −70 • C. Frozen sections were cut from non-tumorous liver and histological examination was performed to verify homogeneous cell p.....
Document: Sampling of human liver tissues was performed as previously described (22) . Seven Chinese patients who had surgical resection of liver at Queen Mary Hospital of Hong Kong were randomly selected for study. All specimens were obtained immediately after surgical resection, snap-frozen in liquid nitrogen and stored at −70 • C. Frozen sections were cut from non-tumorous liver and histological examination was performed to verify homogeneous cell populations of tissues. Hepatitis B surface antigen (HBsAg) was detected in the sera of patients 127, 128, 216 and 229, but not patients 210, 213 and 223. HBV DNA status in liver samples was also verified. The use of human liver tissues was approved by the Joint Institutional Review Board of the University of Hong Kong and Hospital Authority Hong Kong West Cluster.
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