Selected article for: "PCR reaction and quantitative PCR reaction"

Title: Research Communications of the 24th ECVIM-CA Congress
  • Document date: 2015_1_10
  • ID: r59usk02_52
    Snippet: Four myxomatous mitral valves (CKCS) and 4 controls valves were profiled using the Affymetrix Canine Gene 1.0 ST Array. In total 29 out of 302 miRNAs were found to be statistically significantly differentially expressed (down-regulated) based on the false discovery rate, p-value, and fold-change. Expression of three miRNA (cfa-miR-23b, cfa-miR-29c, cfa-miR-218) were also validated by quantitative polymerase chain reaction (Q-PCR, TaqMan), and the.....
    Document: Four myxomatous mitral valves (CKCS) and 4 controls valves were profiled using the Affymetrix Canine Gene 1.0 ST Array. In total 29 out of 302 miRNAs were found to be statistically significantly differentially expressed (down-regulated) based on the false discovery rate, p-value, and fold-change. Expression of three miRNA (cfa-miR-23b, cfa-miR-29c, cfa-miR-218) were also validated by quantitative polymerase chain reaction (Q-PCR, TaqMan), and the results were in agreement with the microarray findings. For network analysis and visualization, Markov clustering algorithms were conducted in Bio-Layout Express 3D , and major clusters of miRNAs were exported and uploaded to the DIANA-miRPath (KEGG pathway) web-server. The pathways identified in the main cluster were attributed to the biological functions of focal adhesion, cytoskeleton (actin) regulation, TGF-b signalling, glycosaminoglycan biosynthesis, osteoclast differentiation, NOTCH signalling and VEGF signalling.

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