Selected article for: "dna extraction and quantitative PCR"
Title: Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017
  • Document date: 2017_11_7
    • ID: roslkxeq_377
    Snippet: Ticks were collected from cats presenting to veterinarians in UK practices. Tick species were identified morphologically and underwent DNA extraction using commercially available kits followed by PCR assays (either conventional or quantitative real-time [qPCR]) for the tick-borne pathogens specified above. All assays had been previously validated for detection of the target species. Feline 28S rDNA served as an endogenous internal PCR control (as.....
    Document: Ticks were collected from cats presenting to veterinarians in UK practices. Tick species were identified morphologically and underwent DNA extraction using commercially available kits followed by PCR assays (either conventional or quantitative real-time [qPCR]) for the tick-borne pathogens specified above. All assays had been previously validated for detection of the target species. Feline 28S rDNA served as an endogenous internal PCR control (assessed within the hemoplasma qPCRs). Additionally, the samples were spiked with an internal amplification control (IAC) to monitor for inhibition. Positive samples from the generic PCRs (Bartonella spp., Hepatozoon spp., Borrelia spp.) were submitted for DNA sequencing for species identification.

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