Author: Sneha Rath; Eliza Prangley; Jesse Donovan; Kaitlin Demarest; Yigal Meir; Ned Wingreen; Alexei Korennykh
Title: Concerted 2-5A-Mediated mRNA Decay and Transcription Reprogram Protein Synthesis in dsRNA Response Document date: 2018_12_4
ID: ng5c7xai_51
Snippet: Magnetic protein A beads were incubated with 2 ïg anti-FLAG (Sigma) or anti-eIF2α (Santa Cruz) antibodies in IP buffer (10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1% Nonidet P-40 (NP-40), 1x complete protease inhibitor cocktail, RNase inhibitor) at 4°C for two hours. Excess unbound antibody was removed by washing the beads twice in IP buffer for 5 minutes. Cells transfected with or without 2-5A for three hours were lysed in 10 mM HEPES (pH 7.5), 10.....
Document: Magnetic protein A beads were incubated with 2 ïg anti-FLAG (Sigma) or anti-eIF2α (Santa Cruz) antibodies in IP buffer (10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1% Nonidet P-40 (NP-40), 1x complete protease inhibitor cocktail, RNase inhibitor) at 4°C for two hours. Excess unbound antibody was removed by washing the beads twice in IP buffer for 5 minutes. Cells transfected with or without 2-5A for three hours were lysed in 10 mM HEPES (pH 7.5), 10 mM NaCl, 2 mM EDTA, 0.5% Triton X-100, 1x complete protease inhibitor cocktail and RNase inhibitor for 7 minutes while rotating, at 4 °C.
Search related documents:
Co phrase search for related documents- mm hepes and ph 10 mm hepes: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- mm hepes and RNase inhibitor: 1, 2, 3, 4
Co phrase search for related documents, hyperlinks ordered by date