Author: Fang, H; Liu, A; Dahmen, U; Dirsch, O
Title: Dual role of chloroquine in liver ischemia reperfusion injury: reduction of liver damage in early phase, but aggravation in late phase Document date: 2013_6_27
ID: qmut89kb_27
Snippet: Gel electrophoresis and western blotting. Twenty micrograms total protein were separated on 12% gels by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinyldifluoride membranes (GE Healthcare, #28906837, Buckinghamshire, UK). Antibodies against phosphorylated or total ERK ( Enzyme-linked immunosorbent assay. Serum HMGB1 was determined with a commercial ELISA assay (Shino-Test, ST51011, Kanagawa, Japan). All proce.....
Document: Gel electrophoresis and western blotting. Twenty micrograms total protein were separated on 12% gels by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinyldifluoride membranes (GE Healthcare, #28906837, Buckinghamshire, UK). Antibodies against phosphorylated or total ERK ( Enzyme-linked immunosorbent assay. Serum HMGB1 was determined with a commercial ELISA assay (Shino-Test, ST51011, Kanagawa, Japan). All procedures were performed according to the instructions of the manufacturer.
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