Selected article for: "genome region and RNA structure"
Author: Valeria Lulla; Andrew E. Firth
Title: A hidden gene in astroviruses encodes a cell-permeabilizing protein involved in virus release
  • Document date: 2019_6_6
    • ID: avq3zwmc_19
    Snippet: The astrovirus subgenomic promoter is also situated within this region of the astrovirus genome ( Fig. 4A ). Although effective replication occurred even in the absence of XP translation (Fig. 4C ), the introduced mutations could still have a potential effect on sgRNA production via alteration of RNA structure and/or other interactions. To map the minimal region required for wt levels of sgRNA production in the context of the pO2RL replicon, we.....
    Document: The astrovirus subgenomic promoter is also situated within this region of the astrovirus genome ( Fig. 4A ). Although effective replication occurred even in the absence of XP translation (Fig. 4C ), the introduced mutations could still have a potential effect on sgRNA production via alteration of RNA structure and/or other interactions. To map the minimal region required for wt levels of sgRNA production in the context of the pO2RL replicon, we gradually truncated the sequence between the start of ORF2 and ORFX. Surprisingly, we found that a replicon containing only the first 4 or 28 nucleotides of ORF2 has very poor subgenomic reporter activity (0.6±0.03 and 12.5±0.1% that of wt pO2RL, respectively). In contrast, including the first 46 nucleotides of ORF2 restored subgenomic reporter activity to 92% of wt (Fig. 4F , S12C). These data suggest that the astrovirus subgenomic promoter is significantly longer than previously reported 19 and extends into the 5′ part ORF2, but does not appear to extend into ORFX.

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