Author: Lin, Mingqun; Liu, Hongyan; Xiong, Qingming; Niu, Hua; Cheng, Zhihui; Yamamoto, Akitsugu; Rikihisa, Yasuko
Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase Document date: 2016_8_19
ID: x5y551c8_44
Snippet: Antibodies against A. phagocytophilum outer membrane protein P44 (mAb 5C11), 124 Localization of native ATG5, LC3, or ubiquitinated proteins was performed with uninfected or E. chaffeensis-infected RF/ 6A cells cultured on coverslips in a 6-well plate at 2 d p.i. Cells were fixed in 4% PFA and labeled with rabbit anti-ATG5 or -LC3B IgG or with mAb FK2 against mono-and poly-ubiquitinated proteins, followed by AF555-conjugated goat anti-rabbit or -.....
Document: Antibodies against A. phagocytophilum outer membrane protein P44 (mAb 5C11), 124 Localization of native ATG5, LC3, or ubiquitinated proteins was performed with uninfected or E. chaffeensis-infected RF/ 6A cells cultured on coverslips in a 6-well plate at 2 d p.i. Cells were fixed in 4% PFA and labeled with rabbit anti-ATG5 or -LC3B IgG or with mAb FK2 against mono-and poly-ubiquitinated proteins, followed by AF555-conjugated goat anti-rabbit or -mouse IgG. DAPI was used to label DNA in the host cell nucleus and E. chaffeensis.
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