Elevated level of renal xanthine oxidase mRNA transcription after nephropathogenic infectious bronchitis virus infection in growing layers

Lin, Huayuan; Huang, Qiqi; Guo, Xiaoquan; Liu, Ping; Liu, Weilian; Zou, Yuelong; Zhu, Shuliang; Deng, Guangfu; Kuang, Jun; Zhang, Caiying; Cao, Huabin; Hu, Guoliang

In addition to the generation of UA, reactive oxygen species (ROS) were also generated by XOD. Chambers et al. [7] reported that XOD was a major source of ROS in reperfused myocardium. In disease promotion and prevention, ROS act as a double-edged sword. On one hand, ROS prevent initiation of disease and play a key role in the immune system and apoptosis to eliminate damaged cells [29] . Vorbach et al. [32] reported that ROS could also function as a microbicide by generating an entire cascade of microbicidal reactions and products. Conversely, the aberrant production of ROS could randomly attack lipids, proteins, nucleic acids, and carbohydrates, causing cell and tissue damage [19] . Former studies have 19reported that overproduction of free radicals could induce excessive degeneration of the polyunsaturated lipids, leading to elevated MDA levels [3, 17] . Previous studies have also shown that MDA was a significant indicator to check lipid peroxidation and cellular oxidative damage [15] , and its concentration was positively correlated with the degree of free radical-induced damage. In the present study, serum MDA values were statistically higher in the infected group than the control group at 8 and 15 dpi (p ＜ 0.01). These results indicated that the chicken suffered oxidative damage induced by the aberrant production of ROS in the infected group.