Selected article for: "extraction kit and nucleic acid"
Author: Shatizadeh, Somayeh; Yavarian, Jila; Rezaie, Farhad; Mahmoodi, Mahmood; Naseri, Maryam; Mokhtari Azad, Talat
Title: Epidemiological and clinical evaluation of children with respiratory virus infections
  • Document date: 2014_9_22
    • ID: yibdumij_5
    Snippet: Nucleic Acid extraction RNA extraction and cDNA synthesis: RNA was extracted from 150 μL of samples using the Neucleospin viral RNA (Neucleospin RNA extraction kit, Macherey-Nagel) according to the manufactures instruction. cDNA synthesis was carried out in 30 μl reaction mixture containing 6 μL of 5X RT Buffer, 2.5 μL of mixed dNTPs (2.5μm each), 1 μL of RT-MULV enzyme (Fermentase), 2.5 μL Random hexamer (Fermentase), 0.5 μL RNase Inhibi.....
    Document: Nucleic Acid extraction RNA extraction and cDNA synthesis: RNA was extracted from 150 μL of samples using the Neucleospin viral RNA (Neucleospin RNA extraction kit, Macherey-Nagel) according to the manufactures instruction. cDNA synthesis was carried out in 30 μl reaction mixture containing 6 μL of 5X RT Buffer, 2.5 μL of mixed dNTPs (2.5μm each), 1 μL of RT-MULV enzyme (Fermentase), 2.5 μL Random hexamer (Fermentase), 0.5 μL RNase Inhibitor (Fermentase) and 17.5 μL RNA template, the mixture was incubated in 22° C for 10 min, 37° C for 45 min and 94° C for 5 min.

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