Author: TERAMICHI, Takurou; FUKUSHI, Shuetsu; HACHIYA, Yuma; MELAKU, Simenew Keskes; OGUMA, Keisuke; SENTSUI, Hiroshi
Title: Evaluation of serological assays available in a biosafety level 2 laboratory and their application for survey of Middle East respiratory syndrome coronavirus among livestock in Ethiopia Document date: 2019_11_5
ID: vijh6x1l_1
Snippet: Pseudovirus VSV-MERS/GFP, a recombinant vesicular stomatitis virus pseudotype, integrated with the GFP gene and coated with the spike protein of MERS-CoV was used in the neutralization test [8] . The infectivity of VSV-MERS/GFP was detected based on GFP expression observed using fluorescence microscopy. GFP-expressing cells in photographic images were counted using VH analyzer. The VSV-MERS/GFP titers were then expressed as focus forming units (F.....
Document: Pseudovirus VSV-MERS/GFP, a recombinant vesicular stomatitis virus pseudotype, integrated with the GFP gene and coated with the spike protein of MERS-CoV was used in the neutralization test [8] . The infectivity of VSV-MERS/GFP was detected based on GFP expression observed using fluorescence microscopy. GFP-expressing cells in photographic images were counted using VH analyzer. The VSV-MERS/GFP titers were then expressed as focus forming units (FFU) per ml.
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