Author: Gaikwad, Satish S.; Lee, Hyun-Jeong; Kim, Ji-Ye; Choi, Kang-Seuk
Title: Expression and serological application of recombinant epitope-repeat protein carrying an immunodominant epitope of Newcastle disease virus nucleoprotein Document date: 2019_1_31
ID: tr3ageky_16
Snippet: Ten NDV negative serum samples from specific pathogen free (SPF) chickens were used to determine a cutoff value for the ELISA assay. The mean negative serum optical density (OD) value plus three standard deviations (SD) was used as the cutoff value. Each sample was repeated in triplicate wells and the mean value was calculated. The experiment was repeated two more times. In addition to SPF chicken serum, different hyper-immune chicken sera to NDV.....
Document: Ten NDV negative serum samples from specific pathogen free (SPF) chickens were used to determine a cutoff value for the ELISA assay. The mean negative serum optical density (OD) value plus three standard deviations (SD) was used as the cutoff value. Each sample was repeated in triplicate wells and the mean value was calculated. The experiment was repeated two more times. In addition to SPF chicken serum, different hyper-immune chicken sera to NDV, H9N2 avian influenza virus (AIV), infectious bronchitis virus (IBV), and infectious bursal disease virus (IBDV) were tested for specificity of rERP using an indirect ELISA assay. To test the sensitivity of the rERP, the chicken anti-NDV serum was diluted serially (1:100-1:6,400) and the serum with each dilution was used to react with the expressed rERP in an indirect ELISA.
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