Author: Narrandes, Shavira; Xu, Wayne
Title: Gene Expression Detection Assay for Cancer Clinical Use Document date: 2018_6_5
ID: wheblwm3_11
Snippet: 17 , 18 qRT-PCR has also been applied to monitor the development of severe acute respiratory syndrome (SARS)-associated coronavirus and detect the prognostic markers in leukemia. 19 In a study establishing a gene signature for human prostate cancer, RNA was extracted from snap-frozen human prostate surgical tissue samples and reverse transcribed using random hexamers and Superscript II Reverse Transcriptase. qRT-PCR then aided in the identificati.....
Document: 17 , 18 qRT-PCR has also been applied to monitor the development of severe acute respiratory syndrome (SARS)-associated coronavirus and detect the prognostic markers in leukemia. 19 In a study establishing a gene signature for human prostate cancer, RNA was extracted from snap-frozen human prostate surgical tissue samples and reverse transcribed using random hexamers and Superscript II Reverse Transcriptase. qRT-PCR then aided in the identification of 8 genes of interest: ornithine decarboxylase, ornithine decarboxylase antizyme, adenosylmethionine decarboxylase, spermidine/spermine N(1)-acetyltransferase, histone H3, growth arrest specific gene, glyceraldehyde 3-phosphate dehydrogenase, and Clusterin. 20 qRT-PCR has also been successfully applied in non-small cell lung cancer (NSCLC) studies to establish a 14-gene signature with 11 cancer-related target genes and 3 reference genes and identify 5 genes essential in the treatment outcome prediction.
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