Author: Narrandes, Shavira; Xu, Wayne
Title: Gene Expression Detection Assay for Cancer Clinical Use Document date: 2018_6_5
ID: wheblwm3_34
Snippet: Primer/probe design FISH requires a DNA probe typically from cloned sources (e.g. plasmids) and target DNA in the form of chromosomes or interphase chromatin that will bind to the probe. The DNA probe can be labelled with haptens, fluorochrome, or dye-based nucleotides. The DNA probe and target DNA are permitted to hybridize to each other only following their denaturation into single strands. Fluorescent microscopy can allow for the visualization.....
Document: Primer/probe design FISH requires a DNA probe typically from cloned sources (e.g. plasmids) and target DNA in the form of chromosomes or interphase chromatin that will bind to the probe. The DNA probe can be labelled with haptens, fluorochrome, or dye-based nucleotides. The DNA probe and target DNA are permitted to hybridize to each other only following their denaturation into single strands. Fluorescent microscopy can allow for the visualization of the DNA probe-target DNA complexes. 71 Similar to FISH, RNA ISH requires a gene-specific nucleotide probe to bind to a certain sequence on a DNA or RNA strand. The probes may be labelled with radio-, antigen-, or fluorescent-labelled bases and analyzed by autoradiography, immunohistochemistry, or fluorescent microscopy, respectively. 58
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