Yeast Kex1p is a Golgi-associated membrane protein: deletions in a cytoplasmic targeting domain result in mislocalization to the vacuolar membrane

Saccharomyces cerevisiae strains c13ABYS86 ($86), Sc25k, and Sc25k-13 (Sc25k + kex/-A/) have been described previously (Cooper and Bussey, 1989) . Other strains used were $6 (a strain sensitive to K1 killer toxin), TCI06cr (MATa leu2 his1 trpl ura3 ), SEYS016a (secl-1 leu2 ura3 gal20), XCY42-30D (MATa ade2-10l ade X [a putative second ade mutation that results in white colonies] urn3 trpl lys2 leu2-3,H2 Amnnl::LEU2), and LB2134-3B (MATa mnng), mnn mutants were crossed with either Sc25 or Sc25-JH (isogenic to Sc25k except MATa). The diploids were sporulated, asci dissected, and spores containing the relevant mutations selected. The resulting strains were HAB595 (mnn/ura3), HAB596 (ura3), and HAB597 (mnn9 ura3).