Author: Bhuiyan, Mejbah Uddin; Snelling, Thomas L; West, Rachel; Lang, Jurissa; Rahman, Tasmina; Borland, Meredith L; Thornton, Ruth; Kirkham, Lea-Ann; Sikazwe, Chisha; Martin, Andrew C; Richmond, Peter C; Smith, David W; Jaffe, Adam; Blyth, Christopher C
Title: Role of viral and bacterial pathogens in causing pneumonia among Western Australian children: a case–control study protocol Document date: 2018_3_16
ID: w3rxdaii_46
Snippet: After collection, the NPS specimen is kept on ice and processed within 4h at the research laboratory of the PMH Children's Clinical Research Facility (CCRF). The NPS specimen is vortexed for 30 seconds before divided into two aliquots with 500 µl each for bacterial and viral assessment, and the vials stored at -80°C. Specimens are batched and periodically sent to reference laboratories for PCR testing. The NPS specimens are transported to the r.....
Document: After collection, the NPS specimen is kept on ice and processed within 4h at the research laboratory of the PMH Children's Clinical Research Facility (CCRF). The NPS specimen is vortexed for 30 seconds before divided into two aliquots with 500 µl each for bacterial and viral assessment, and the vials stored at -80°C. Specimens are batched and periodically sent to reference laboratories for PCR testing. The NPS specimens are transported to the reference laboratories on ice on the same day of testing to avoid an additional freeze-thaw cycle.
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