Selected article for: "PCR product and virus presence"
Author: Ma, Ge; Greenwell-Wild, Teresa; Lei, Kejian; Jin, Wenwen; Swisher, Jennifer; Hardegen, Neil; Wild, Carl T.; Wahl, Sharon M.
Title: Secretory Leukocyte Protease Inhibitor Binds to Annexin II, a Cofactor for Macrophage HIV-1 Infection
  • Document date: 2004_11_15
    • ID: rlabxfss_23_1
    Snippet: -based assay. The presence of anti-annexin II during the initial virus inoculation period dramatically inhibited subsequent viral DNA synthesis, as demonstrated by the HIV-1-specific 730-bp PCR product (Fig. 5 E) , even though annexin II blockade did not disengage the viral-binding step (Fig. 5 C) . Collectively, these data indicate that interference with macrophage membrane annexin II inhibits infectivity after binding, but pre-reverse transcrip.....
    Document: -based assay. The presence of anti-annexin II during the initial virus inoculation period dramatically inhibited subsequent viral DNA synthesis, as demonstrated by the HIV-1-specific 730-bp PCR product (Fig. 5 E) , even though annexin II blockade did not disengage the viral-binding step (Fig. 5 C) . Collectively, these data indicate that interference with macrophage membrane annexin II inhibits infectivity after binding, but pre-reverse transcription, consistent with a stranglehold on the viral entry/fusion step.

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