Sendai virus intra-host population dynamics and host immunocompetence influence viral virulence during in vivo passage

Peña, José; Chen-Harris, Haiyin; Allen, Jonathan E.; Hwang, Mona; Elsheikh, Maher; Mabery, Shalini; Bielefeldt-Ohmann, Helle; Zemla, Adam T.; Bowen, Richard A.; Borucki, Monica K.

In the secondary host transmission study, unpassaged SeV seed stock, PBS (Mock) and the SeV virus obtained from P10 BAL of mice in each diet group, were used to infect a different host, guinea pigs, under ND conditions (three animals per treatment group). None of the guinea pigs lost weight or displayed any signs of clinical infection; however, guinea pigs infected with P10 BAL from the ND and SD mouse groups had significantly higher lung histopathology scores than those infected with BAL from the FD mouse group or seed stock, which showed virtually no histopathology changes (Fig. 2C ).