Sendai virus intra-host population dynamics and host immunocompetence influence viral virulence during in vivo passage

Peña, José; Chen-Harris, Haiyin; Allen, Jonathan E.; Hwang, Mona; Elsheikh, Maher; Mabery, Shalini; Bielefeldt-Ohmann, Helle; Zemla, Adam T.; Bowen, Richard A.; Borucki, Monica K.

High-frequency variants were also detected at nt 8,053, 8,073, and 8,265 of the HN gene (Figs. 5A-C and 6A-C, Supplementary Table S2 ). An A454V consensus mutation was detected in the BAL sample from SD.GP2. At this same site, nt Values are means 6 SEM; n ¼ 4 for mock infected and n ¼ 5 for ND, SD, and FD. Statistical significance was determined by unpaired t-test and considered significant if P < 0.05. Cytokines to the left of the hash line correspond to pg/ml y-axis and cytokines to the right of the hash line correspond to the MFI y-axis. ND, normal diet; FD, fat diet; SD, selenium deficient; MFI, mean fluorescence intensity. 8053, two variants were identified at different frequencies. The more prevalent change was a C to T with a range of 3-31% was detected across all diet groups in the P1, P10, and guinea pig samples. This mutation was detected at a 6.5% in the seed inoculum, thus it appears to have been selected for during passage.