Author: Angelini, Megan M.; Akhlaghpour, Marzieh; Neuman, Benjamin W.; Buchmeier, Michael J.
Title: Severe Acute Respiratory Syndrome Coronavirus Nonstructural Proteins 3, 4, and 6 Induce Double-Membrane Vesicles Document date: 2013_8_13
ID: yl1qyh7j_21
Snippet: Immunofluorescence assays. HEK293T cells were grown on poly-Llysine-coated coverslips, transfected, and fixed 24 h posttransfection using 3.7% paraformaldehyde, permeabilized with 0.1% Triton X-100, and mounted with DAPI (4=,6-diamidino-2-phenylindole) Fluoromount D (Southern Biotech). Confocal microscopy was performed with a Nikon Eclipse Ti confocal microscope. Images were processed using NIS Elements software......
Document: Immunofluorescence assays. HEK293T cells were grown on poly-Llysine-coated coverslips, transfected, and fixed 24 h posttransfection using 3.7% paraformaldehyde, permeabilized with 0.1% Triton X-100, and mounted with DAPI (4=,6-diamidino-2-phenylindole) Fluoromount D (Southern Biotech). Confocal microscopy was performed with a Nikon Eclipse Ti confocal microscope. Images were processed using NIS Elements software.
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