Author: Lan, Han-hong; Wang, Cui-mei; Chen, Shuang-shuang; Zheng, Jian-ying
Title: siRNAs Derived from Cymbidium Mosaic Virus and Odontoglossum Ringspot Virus Down-modulated the Expression Levels of Endogenous Genes in Phalaenopsis equestris Document date: 2019_10_1
ID: qgrhh3r4_7
Snippet: We obtained the sequences of the RNAi pathway core components in the genome of P. equestris through a local BLASTx search with corresponding sequences of Oryza sativa as queries (Cai et al., 2015; Kapoor et al., 2008; Niu et al., 2016) . Furthermore, we used the sRNA target analysis server psRNATarget (Dai and Zhao, 2011) to predict putative target genes of P. equestris by CymMV-and ORSV-derived siRNAs. We then investigated the transcript levels .....
Document: We obtained the sequences of the RNAi pathway core components in the genome of P. equestris through a local BLASTx search with corresponding sequences of Oryza sativa as queries (Cai et al., 2015; Kapoor et al., 2008; Niu et al., 2016) . Furthermore, we used the sRNA target analysis server psRNATarget (Dai and Zhao, 2011) to predict putative target genes of P. equestris by CymMV-and ORSV-derived siRNAs. We then investigated the transcript levels of these core components and target genes by RT-qPCR assay on a Quant-Studio 6 Flex Real-Time PCR System (Life Technologies, Carlsbad, CA, USA) using the SYBR Green PCR Master-Mix kit (Promega, Madison, WI, USA) with primers (Table 1) as described previously (Lan et al., 2015 (Lan et al., , 2018a .
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