Author: Blank, Maximilian F.; Chen, Sifan; Poetz, Fabian; Schnölzer, Martina; Voit, Renate; Grummt, Ingrid
Title: SIRT7-dependent deacetylation of CDK9 activates RNA polymerase II transcription Document date: 2017_3_17
ID: qm9urt2w_45
Snippet: To examine whether SIRT7 also activates P-TEFb by facilitating the release of CDK9/cyclin T1 from the inhibitory HEXIM1/7SK ribonucleoprotein complex, we incubated bead-bound P-TEFb/7SK snRNP complexes with Flag-SIRT7 and monitored the release of P-TEFb on immunoblots. As shown in Figure 3I , wildtype SIRT7 but not the catalytically inactive point mutant promoted release of P-TEFb from the 7SK snRNP complex in an NAD +dependent manner (see also S.....
Document: To examine whether SIRT7 also activates P-TEFb by facilitating the release of CDK9/cyclin T1 from the inhibitory HEXIM1/7SK ribonucleoprotein complex, we incubated bead-bound P-TEFb/7SK snRNP complexes with Flag-SIRT7 and monitored the release of P-TEFb on immunoblots. As shown in Figure 3I , wildtype SIRT7 but not the catalytically inactive point mutant promoted release of P-TEFb from the 7SK snRNP complex in an NAD +dependent manner (see also Supplementary Figure S3I ). Together these results indicate that SIRT7 mediates CTD-Ser2 phosphorylation and transcription elongation both by activation of CDK9 and releasing P-TEFb from the 7SK snRNP complex.
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