Author: Liu, Yuan-yuan; Chen, Liang-jun; Zhong, Yan; Shen, Meng-xin; Ma, Nian; Liu, Bing-yu; Luo, Fan; Hou, Wei; Yang, Zhan-qiu; Xiong, Hai-rong
Title: Specific interference shRNA-expressing plasmids inhibit Hantaan virus infection in vitro and in vivo Document date: 2016_3_14
ID: pwlybr2h_23
Snippet: Immunofluorescence assay (IFA) IFA was performed to detect the hantavirus antigens in the infected Vero-E6 cells as previously reported [18] with monoclonal antibody A35, which targets the nucleocapsid protein (1:200 dilution, provided by the Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China), or rabbit polyclonal antibody targeting glycoprotein G2 (1:100 dilution, provided by Prof John W HUGGINS, Virology Division, Un.....
Document: Immunofluorescence assay (IFA) IFA was performed to detect the hantavirus antigens in the infected Vero-E6 cells as previously reported [18] with monoclonal antibody A35, which targets the nucleocapsid protein (1:200 dilution, provided by the Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China), or rabbit polyclonal antibody targeting glycoprotein G2 (1:100 dilution, provided by Prof John W HUGGINS, Virology Division, United States Army Medical Research Institute of Infectious Disease). After incubation, FITC-conjugated goat anti-mouse or anti-rabbit IgG (Sigma Aldrich, 1:200 dilution) secondary antibodies were used. The cell cytoplasm was stained red with Evans blue. The images were collected using a fluorescence microscope (Nikon TE2000).
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